Coding
DcuC-NS

Part:BBa_K2385000:Design

Designed by: Josine oude Lohuis   Group: iGEM17_Amsterdam   (2017-09-26)


DcuC (N. Spumigena)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DcuC-NS contained 6 restriction sites that had to be removed (on position 555, 732, 1011, 1119, 1143 and 1263). For each case, one base was changed in a way that the amino acid composition remained the same and the codon had roughly the same frequency in our model organism (Syenchocystis).

Furthermore, we have codon optimised the gene for Synechocystis and to this and changed 15 bases.


Source

This gene was obtained from the NCBI gene bank.

>AHJ29173.1 C4-dicarboxylate transporter DcuC [Nodularia spumigena CCY9414] MLSTFAPLVIILIAMIFMFKRVDVRLSLGLSATGLFLIAGKLPQLFVTITQQMTNEKTVVPICTAMGFAY VLRLTECDRHLTHLLLAPLRHGRWLLIPGGIIAAYIVNMAIVSQSSTAAIVGTVLLPLLLAVNITPVIAG SLLLLGSSMGGELFNPGAVEIVKLAELTGQPVAKLVAQVLPINLLASITTLIVFCILAVILSQKAVLLSP EITKVDSDVAQSGKPFHLNLIKALVPLLPLALLFIIPALVQLPKEFSNNSVSIAAAMLIAVTAAGLTTPK ESGGLVAAFFEGAGFAYANIISIIIVATIFTDGIKANGLIEVLRNALANRPVAVKITSLILPFTLAGVTG SGSAPAIAVMNVLVPVATTMNLDPVKIGALAAVAAQLGRTLSPVAAVVIMSATISKQPPLNLVKCVVLPL LAGLAAVIVATLCNWV

References